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Polymersomes: Third‐Party ATP Sensing in Polymersomes: A Label‐Free Assay of Enzyme Reactions in Vesicular Compartments (Small 3/2014)
Author(s) -
Yildiz Umit Hakan,
De Hoog HansPeter M.,
Fu Zhikang,
Tomczak Nikodem,
Parikh Atul N.,
Nallani Madhavan,
Liedberg Bo
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201470019
Subject(s) - polymersome , adenosine triphosphate , chemistry , atp hydrolysis , enzyme , fluorescence , quenching (fluorescence) , biochemistry , biophysics , alkaline phosphatase , polymer , biology , atpase , organic chemistry , physics , quantum mechanics , copolymer , amphiphile
Polymersomes are encapsulated with a fluorescent reporter and a non‐labeled enzyme for sensing of adenosine triphosphate (ATP). As B. Liedberg and co‐workers report on page 442, passive diffusion of exogenously added ATP through the membrane is sensed by monitoring the ATPinduced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter‐bound ATP by alkaline phosphatase.