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Surface‐Tunable Bioluminescence Resonance Energy Transfer via Geometry‐Controlled ZnO Nanorod Coordination
Author(s) -
Lim Jun Hyung,
Park Geun Chul,
Lee Seung Muk,
Lee Jung Heon,
Lim Butaek,
Hwang Soo Min,
Kim Jung Ho,
Park Hansoo,
Joo Jinho,
Kim YoungPil
Publication year - 2015
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201403700
Subject(s) - nanorod , bioluminescence , energy transfer , förster resonance energy transfer , materials science , surface (topology) , nanotechnology , resonance (particle physics) , chemical engineering , chemical physics , chemistry , geometry , fluorescence , optics , atomic physics , physics , biochemistry , mathematics , engineering
The use of ZnO nanorods (NRs) as an effective coordinator and biosensing platform to create bioluminescence resonance energy transfer (BRET) is reported. Herein, a hydrothermal approach is applied to obtain morphologically controlled ZnO NRs, which are directly bound to luciferase (Luc) and carboxy‐modified quantum dot (QD) acting as a donor–acceptor pair for BRET. BRET efficiency varies significantly with the geometry of ZnO NRs, which modulates the coordination between hexahistidine‐tagged Luc (Luc‐His 6 ) and QD, owing to the combined effect of the total surface area consisting of (001) and (100) planes and their surface polarities. Unlike typical QD–BRET reactions with metal ions (e.g., zinc ions), a geometry‐controlled ZnO NR platform can facilitate the design of surface‐initiated BRET sensors without being supplemented by copious metal ions: the geometry‐controlled ZnO NR platform can therefore pave the way for nanostructure‐based biosensors with enhanced analytical performance.