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Microfluidic Single‐Cell Analysis with Affinity Beads
Author(s) -
Werner Michael,
Palankar Raghavendra,
Arm Loïc,
Hovius Ruud,
Vogel Horst
Publication year - 2015
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201402650
Subject(s) - lysis , microfluidics , phagosome , bead , single cell analysis , intracellular , analyte , phagocytosis , cell , micrometer , biophysics , chemistry , nanotechnology , materials science , chromatography , biochemistry , microbiology and biotechnology , biology , optics , physics , composite material
A micrometer‐sized affinity bead (red) is (i) taken up into a cell by phagocytosis, (ii) photochemically released from phagosomes, (iii) optically trapped by the cell, and (iv) isolated by cell lysis for subsequent analysis of captured intracellular analyte (green).