Premium
Inorganic–Organic Hybrid Nanoprobe for NIR‐Excited Imaging of Hydrogen Sulfide in Cell Cultures and Inflammation in a Mouse Model
Author(s) -
Zhou Yi,
Chen Wangqiao,
Zhu Jixin,
Pei Wenbo,
Wang Chengyuan,
Huang Ling,
Yao Cheng,
Yan Qinyu,
Huang Wei,
Loo Joachim Say Chye,
Zhang Qichun
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201401867
Subject(s) - nanoprobe , photon upconversion , fluorescence , hydrogen sulfide , materials science , nanotechnology , luminescence , chemistry , biophysics , nanoparticle , optoelectronics , sulfur , physics , metallurgy , quantum mechanics , biology
Hydrogen sulfide (H 2 S) is an important gaseous signaling agent mediated by many physiological processes and diseases. In order to explore its role in biological signaling, much effort has been focused on developing organic fluorescent probes to image H 2 S. However, these downconversion H 2 S probes are impractical for bio‐imaging beyond a certain depth because of the short tissue penetration of UV/visible light (as an excitation source). In most circumstance, these probes are also not suitable for long‐term assay due to photo‐bleaching. Herein, a new design to detect H 2 S based on the coumarin‐hemicyanine (CHC1)‐modified upconversion nanophosphors is reported. This inorganic–organic integrated nanoprobe is demonstrated to display a fast response time with a large ratiometric upconversion luminescence (UCL) enhancement, and extraordinary photo‐stability. CHC1‐UCNPs not only can be used for ratiometric UCL monitoring of pseudo‐enzymatic H 2 S production in living cells, but can also be used to identify the risk of endotoxic shock through ratiometric UCL imaging of tissue and measurement of endogenous H 2 S levels in plasma. The first ratiometric UCL H 2 S nanoprobe reported here may be further developed as the next‐generation diagnostic tool for the detection of inflammatory‐related diseases.