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A Fluorescent Nucleic Acid Nanodevice Quantitatively Images Elevated Cyclic Adenosine Monophosphate in Membrane‐Bound Compartments
Author(s) -
Sharma Suruchi,
Zaveri Anisha,
Visweswariah Sandhya S.,
Krishnan Yamuna
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201400833
Subject(s) - nanodevice , nucleic acid , fluorescence , computer science , chemistry , biophysics , adenosine , membrane , nanotechnology , biochemistry , biology , materials science , physics , optics
cAMPhor: In the presence of cAMP, cAMPhor folds into a structure that binds DFHBI (green), increasing its fluorescence, while Alexa 647 (red) functions as a normalizing dye. It can thus be used to spatially image cAMP quantitatively in membrane‐bound compartments.