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Spatially Selecting a Single Cell for Lysis Using Light‐Induced Electric Fields
Author(s) -
Witte Christian,
Kremer Clemens,
Chanasakulniyom Mayuree,
Reboud Julien,
Wilson Rab,
Cooper Jonathan M.,
Neale Steven L.
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201400247
Subject(s) - lysis , microfluidics , lysis buffer , materials science , nanotechnology , biophysics , optoelectronics , chemistry , biology , biochemistry
An optoelectronic tweezing (OET) device , within an integrated microfluidic channel, is used to precisely select single cells for lysis among dense populations. Cells to be lysed are exposed to higher electrical fields than their neighbours by illuminating a photoconductive film underneath them. Using beam spot sizes as low as 2.5 μm, 100% lysis efficiency is reached in <1 min allowing the targeted lysis of cells.