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Cellular Uptake: Uptake Kinetics and Nanotoxicity of Silica Nanoparticles Are Cell Type Dependent (Small 23/2013)
Author(s) -
Blechinger Julia,
Bauer Alexander T.,
Torrano Adriano A.,
Gorzelanny Christian,
Bräuchle Christoph,
Schneider Stefan W.
Publication year - 2013
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201370142
Subject(s) - nanotoxicology , nanoparticle , fluorescence microscope , biophysics , kinetics , intracellular , fluorescence , nanotechnology , materials science , microscopy , homogeneous , chemistry , biochemistry , biology , optics , physics , quantum mechanics , thermodynamics
Three‐dimensional atomic force microscopy is combined with fluorescence microscopy to investigate the cellular uptake of silica nanoparticles into human cells. As reported on page 3970 by C. Bräuchle, S. W. Schneider, and co‐workers, nanoparticles (red dots in the image) are visible on the plasma membrane outside of the cells. Interestingly, after longer exposure times, the surface of the cells may also be characterized by homogeneous distribution of small humps, indicating intracellular localization of nanoparticles. Results obtained using this technique confirm that the cellular response to silica nanoparticles is cell type dependent.