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Microfluidics: Force‐Controlled Fluidic Injection into Single Cell Nuclei (Small 11/2013)
Author(s) -
GuillaumeGentil Orane,
Potthoff Eva,
Ossola Dario,
Dörig Pablo,
Zambelli Tomaso,
Vorholt Julia A.
Publication year - 2013
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201370061
Subject(s) - microfluidics , fluidics , cantilever , pyramid (geometry) , materials science , atomic force microscopy , nanotechnology , nucleus , indentation , cell , fluorescence microscope , biophysics , fluorescence , chemistry , microbiology and biotechnology , optics , composite material , biochemistry , physics , aerospace engineering , engineering , biology
Fluidic force microscopy for the injection of femtoliter volumes into single cell nuclei is described by T. Zambelli, J. A. Vorholt, and co‐workers on page 1904 . The pyramid of a microfluidic AFM cantilever is precisely inserted into the cell nucleus under force control, while a solution loaded in the probe is delivered to the nuclear compartment through the application of a pressure pulse. Efficient injection with simultaneous recording of cell indentation events, as well as tunable volume release, are demonstrated. Cell transformation by injection of plasmid DNA is validated by gfp expression.