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Upconversion Luminescence Resonance Energy Transfer (LRET)‐Based Biosensor for Rapid and Ultrasensitive Detection of Avian Influenza Virus H7 Subtype
Author(s) -
Ye Wei Wei,
Tsang MingKiu,
Liu Xuan,
Yang Mo,
Hao Jianhua
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201303766
Subject(s) - biosensor , hemagglutinin (influenza) , förster resonance energy transfer , detection limit , oligonucleotide , photon upconversion , covalent bond , quenching (fluorescence) , aptamer , molecular beacon , materials science , luminescence , chemistry , fluorescence , nanotechnology , virus , virology , microbiology and biotechnology , biology , gene , biochemistry , optoelectronics , chromatography , physics , organic chemistry , quantum mechanics
Avian influenza viruses (AIV) with good adaptation and various mutations have threatened both human and animals’ health. The H7 subtypes have the potential to cause pandemic threats to human health due to the highly pathogenic characteristics. Therefore, it is quite urgent to develop a novel biosensor for rapid and sensitive detection of H7 subtypes. In this work, a biosensor based on luminescence resonance energy transfer (LRET) from BaGdF 5 :Yb/Er upconversion nanoparticles (UCNPs) to gold nanoparticles (AuNPs) has been developed for rapid and sensitive H7 subtypes detection. The amino modified capture oligonucleotide probes are covalently linked to poly(ethylenimine) (PEI) modified BaGdF 5 :Yb/Er UCNPs. The thiol modified oligonucleotides with H7 hemagglutinin gene sequence are conjugated to surfaces of AuNPs. The hybridization process between complementary strands of H7 Hemagglutinin gene and its probe brings the energy donor and acceptor into close proximity, leading to the quenching of fluorescence of UCNPs. A linear response is obtained ranging from 10 p m to 10 n m and the limit of detection (LOD) is around 7 p m with detection time around 2 hours. This biosensor is expected to be a valuable diagnostic tool for rapid and sensitive detection of AIV.