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Synthesis of Multi‐Fullerenes Encapsulated Palladium Nanocage, and Its Application in Electrochemiluminescence Immunosensors for the Detection of Streptococcus suis Serotype 2
Author(s) -
Wang Haijun,
Bai Lijuan,
Chai Yaqin,
Yuan Ruo
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201303594
Subject(s) - electrochemiluminescence , nanocages , materials science , fullerene , nanotechnology , chemistry , detection limit , catalysis , chromatography , biochemistry , organic chemistry
A novel functionalized material is synthesized using surface‐decorated fullerene (C 60 ) to encapsulate hollow and porous palladium nanocages (PdNCs), and is applied to fabricate an electrochemiluminescence (ECL) immunosensor for the detection of Streptococcus suis Serotype 2 (SS2). PdNCs with hollow interiors and porous walls are prepared using a galvanic replacement reaction between silver nanocubes and metal precursor salts. Then, C 60 reacts with l ‐cysteine ( l ‐Cys) to form l ‐Cys functionalized C 60 (C 60 ‐ l ‐Cys), which has a better biocompatibility, conductivity, and hydrophilicity compared to C 60 and possesses abundant –SH groups on the surface. Because of the special interaction between –SH and PdNCs, the obtained C 60 ‐ l ‐Cys is adsorbed around the PdNCs to form an interesting structure with multiple spheres encapsulating the cage. The resultant functionalized material (C 60 ‐L‐Cys‐PdNCs) has a high specific surface area, good electrocatalytic ability, and efficient photocatalytic activity, and is used to construct an ECL immunosensor for the detection of SS2. The ECL signal amplified strategy is performed by using the novel coreactant (C 60 ‐ l ‐Cys) and in situ generation of O 2 thus creating the S 2 O 8 2− ‐O 2 ECL system. As a result, a wide linear detection range of 0.1 pg mL −1 to 100 ng mL −1 is acquired with a relatively low detection limit of 33.3 fg mL −1 .