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Nanostructured Polymeric Coatings Based on Chitosan and Dopamine‐Modified Hyaluronic Acid for Biomedical Applications
Author(s) -
Neto Ana I.,
Cibrão Ana C.,
Correia Clara R.,
Carvalho Rita R.,
Luz Gisela M.,
Ferrer Gloria G.,
Botelho Gabriela,
Picart Catherine,
Alves Natália M.,
Mano João F.
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201303568
Subject(s) - quartz crystal microbalance , adhesion , chitosan , contact angle , materials science , adhesive , layer by layer , chemical engineering , catechol , horseradish peroxidase , hyaluronic acid , layer (electronics) , nanotechnology , chemistry , organic chemistry , composite material , adsorption , engineering , enzyme , biology , genetics
In a marine environment, specific proteins are secreted by mussels and used as a bioglue to stick to a surface. These mussel proteins present an unusual amino acid 3,4‐dihydroxyphenylalanine (known as DOPA). The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA. Inspired by the structure and composition of these adhesive proteins, dopamine‐modified hyaluronic acid (HA‐DN) prepared by carbodiimide chemistry is used to form thin and surface‐adherent dopamine films. This conjugate was characterized by distinct techniques, such as nuclear magnetic resonance and ultraviolet spectrophotometry. Multilayer films are developed based on chitosan and HA‐DN to form polymeric coatings using the layer‐by‐layer methodology. The nanostructured films formation is monitored by quartz crystal microbalance. The film surface is characterized by atomic force microscopy and scanning electron microscopy. Water contact angle measurements are also conducted. The adhesion properties are analyzed showing that the nanostructured films with dopamine promote an improved adhesion. In vitro tests show an enhanced cell adhesion, proliferation and viability for the biomimetic films with catechol groups, demonstrating their potential to be used in distinct biomedical applications.