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Super‐Resolution Biological Microscopy Using Virtual Imaging by a Microsphere Nanoscope
Author(s) -
Yang Hui,
Moullan Norman,
Auwerx Johan,
Gijs Martin A. M.
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201302942
Subject(s) - microsphere , nanoscopic scale , resolution (logic) , materials science , nanostructure , microscopy , refractive index , microscope , diffraction , image resolution , optics , nanotechnology , optical microscope , near field scanning optical microscope , scanning electron microscope , optoelectronics , physics , computer science , chemical engineering , artificial intelligence , engineering
Super‐resolution imaging of sub‐cellular organelles and nanostructures is achieved by simply putting high‐refractive index glass microspheres on a sample in combination with a microscope immersion objective for observation. The microsphere projects the sample's near‐field nanoscopic features into the far‐field, generating a magnified virtual image and demonstrating a resolution four times better than that corresponding to the classical diffraction limit.