Premium
In Vivo Volumetric Photoacoustic Molecular Angiography and Therapeutic Monitoring with Targeted Plasmonic Nanostars
Author(s) -
Nie Liming,
Wang Shouju,
Wang Xiaoyong,
Rong Pengfei,
Ma Ying,
Liu Gang,
Huang Peng,
Lu Guangming,
Chen Xiaoyuan
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201302924
Subject(s) - materials science , photoacoustic imaging in biomedicine , photothermal therapy , penetration depth , laser , biomedical engineering , molecular imaging , in vivo , homogeneous , preclinical imaging , optics , nanotechnology , medicine , physics , microbiology and biotechnology , biology , thermodynamics
Photoacoustic (PA) imaging promises deeper tissue penetration while maintaining rich optical contrast as compared to other high resolution optical imaging techniques. In this report, a near‐infrared pulse laser serves as the excitation source, and 128 ultrasonic transducers are spirally distributed on a hemispherical surface to receive PA signals for three‐dimensional (3D) image reconstruction. With these attributes, the unique modality produces an isotropic and homogeneous spatial resolution (∼200 μm) with penetration depth of centimeters. Cyclic Arg‐Gly‐Asp (RGD) peptides conjugated plasmonic gold nanostars (RGD‐GNS) are designed to specifically target over‐expressed integrin α v β 3 on tumor neovasculature, enabling highly sensitive angiography and photothermal therapy (PTT). After the administration of RGD‐GNS, tumor angiogenesis is clearly imaged with enhanced contrast, and the growth of tumor is effectively inhibited by PTT after laser irradiation. This study suggest that the PA angiography with plasmonic RGD‐GNS can be applied as a triple functional platform for tumor diagnosis, PTT, and treatment monitoring. This PA technique offers deeper imaging depth with homogeneous resolution over existing optical imaging techniques for early diagnosis of tumor angiogenesis as well as on‐the‐spot nanotherapeutic evaluation.