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Benzoboroxole‐Functionalized Magnetic Core/Shell Microspheres for Highly Specific Enrichment of Glycoproteins under Physiological Conditions
Author(s) -
Zhang Yuting,
Ma Wanfu,
Li Dian,
Yu Meng,
Guo Jia,
Wang Changchun
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201302841
Subject(s) - microsphere , glycoprotein , core (optical fiber) , materials science , shell (structure) , nanotechnology , biophysics , chemical engineering , chemistry , biochemistry , composite material , biology , engineering
Efficient enrichment of specific glycoproteins from complex biological samples is of great importance towards the discovery of disease biomarkers in biological systems. Recently, phenylboronic acid‐based functional materials have been widely used for enrichment of glycoproteins. However, such enrichment was mainly carried out under alkaline conditions, which is different to the status of glycoproteins in neutral physiological conditions and may cause some unpredictable degradation. In this study, on‐demand neutral enrichment of glycoproteins from crude biological samples is accomplished by utilizing the reversible interaction between the cis ‐diols of glycoproteins and benzoboroxole‐functionalized magnetic composite microspheres (Fe 3 O 4 /PAA‐AOPB). The Fe 3 O 4 /PAA‐AOPB composite microspheres are deliberately designed and constructed with a high‐magnetic‐response magnetic supraparticle (MSP) core and a crosslinked poly(acrylic acid) (PAA) shell anchoring abundant benzoboroxole functional groups on the surface. These nanocomposites possessed many merits, such as large enrichment capacity (93.9 mg/g, protein/beads), low non‐specific adsorption, quick enrichment process (10 min) and magnetic separation speed (20 s), and high recovery efficiency. Furthermore, the as‐prepared Fe 3 O 4 /PAA‐AOPB microspheres display high selectivity to glycoproteins even in the E. coli lysate or fetal bovine serum, showing great potential in the identify of low‐abundance glycoproteins as biomarkers in real complex biological systems for clinical diagnoses.