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Ultrasensitive and Closed‐Tube Colorimetric Loop‐Mediated Isothermal Amplification Assay Using Carboxyl‐Modified Gold Nanoparticles
Author(s) -
Wong Jacky K. F.,
Yip Shea Ping,
Lee Thomas M. H.
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201302348
Subject(s) - loop mediated isothermal amplification , naked eye , colloidal gold , materials science , nanoparticle , pyrophosphate , isothermal process , ion , colorimetry , tube (container) , particle (ecology) , dna , chemistry , detection limit , nanotechnology , chromatography , organic chemistry , biochemistry , physics , oceanography , geology , composite material , thermodynamics , enzyme
LAMP ends up in purple/red : The incorporation of 11‐mercaptoundecanoic acid‐modified gold nanoparticles into loop‐mediated isothermal amplification (LAMP) reaction enables as few as 200 copies of a target DNA sequence to be detected by the naked eye. The color is controlled by magnesium ion‐templated aggregation, and in the presence of the target, the LAMP reaction by‐product pyrophosphate ion leads to particle deaggregation.