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Probing Physical Properties of a DNA‐Protein Complex Using Nanofluidic Channels
Author(s) -
Frykholm Karolin,
Alizadehheidari Mohammadreza,
Fritzsche Joachim,
Wigenius Jens,
Modesti Mauro,
Persson Fredrik,
Westerlund Fredrik
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201302028
Subject(s) - dna , nanotechnology , passivation , protein filament , computer science , materials science , chemistry , layer (electronics) , biochemistry
A method to investigate physical properties of a DNA‐protein complex in solution is demonstrated. By using tapered nanochannels and lipid passivation the persistence length of a RecA filament formed on double‐stranded DNA is determined to 1.15 μm, in agreement with the literature, without attaching protein or DNA to any handles or surfaces.