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Ultrathin Shell Double Emulsion Templated Giant Unilamellar Lipid Vesicles with Controlled Microdomain Formation
Author(s) -
Arriaga Laura R.,
Datta Sujit S.,
Kim ShinHyun,
Amstad Esther,
Kodger Thomas E.,
Monroy Francisco,
Weitz David A.
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201301904
Subject(s) - lipid microdomain , vesicle , membrane , emulsion , microfluidics , materials science , fabrication , nanotechnology , chemical engineering , chemistry , medicine , biochemistry , alternative medicine , pathology , engineering
A microfluidic approach is reported for the high‐throughput, continuous production of giant unilamellar vesicles (GUVs) using water‐in‐oil‐in‐water double emulsion drops as templates. Importantly, these emulsion drops have ultrathin shells; this minimizes the amount of residual solvent that remains trapped within the GUV membrane, overcoming a major limitation of typical microfluidic approaches for GUV fabrication. This approach enables the formation of microdomains, characterized by different lipid compositions and structures within the GUV membranes. This work therefore demonstrates a straightforward and versatile approach to GUV fabrication with precise control over the GUV size, lipid composition and the formation of microdomains within the GUV membrane.