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Reduced Graphene Oxide Upconversion Nanoparticle Hybrid for Electrochemiluminescent Sensing of a Prognostic Indicator in Early‐Stage Cancer
Author(s) -
Wu Li,
Wang Jiasi,
Yin Meili,
Ren Jinsong,
Miyoshi Daisuke,
Sugimoto Naoki,
Qu Xiaogang
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201301273
Subject(s) - cancer cell , cyclin dependent kinase , cancer research , nanotechnology , materials science , cdk inhibitor , cancer , cyclin , chemistry , cell cycle , cell , biology , biochemistry , genetics
Upconversion nanoparticles (UCNPs) have been proposed as a promising new class of biological luminescent labels because of their weak auto‐fluorescence background, strong penetration ability under near‐infrared (NIR) radiation, resistance to photobleaching, and low toxicity. Although UCNPs hold great promise in nanotechnology and nanomedicine, their applications in ECL fields still remain unexplored. Herein, a label‐free, ultra‐sensitive and selective electrochemiluminescence (ECL) assay is developed for detection of cyclin A 2 by using highly efficient ECL graphene‐upconversion hybrid. Being an important member of the cyclin family, cyclin A 2 is involved in the initiation of DNA replication, transcription and cell cycle regulation through the association of cyclin‐dependent kinases (CDK). Cyclin A 2 is a prognostic indicator in early‐stage cancers and a target for treatment of different types of cancers. However, the expression level of cyclin A 2 is quite low, direct detection of cyclin A 2 in crude cancer cell extracts is challenging and important for both clinical diagnosis of cancer in the early stage and the treatment. By chemically grafting cyclin A 2 detection specific probe, a PEGlyted hexapeptide, to graphene‐upconversion hybrid, the constructed ECL biosensor displays a superior performance for cyclin A 2 , which can not only detect cyclin A 2 directly in cancer cell extracts, but also discriminate between normal cells and cancer cells. More importantly, the ECL biosensor has different responses between clinical used anticancer drug‐treated and non‐treated cancer cells, which demonstrates that the sensor can be potentially used for drug screening, and for evaluation of therapeutic treatments in early‐stage cancers.