Premium
β‐D‐Glucosidase Assisted Gold Dissolution as Non‐Optical and Quantifiable Detection Technique for Immunoassays
Author(s) -
Koehler F. M.,
Raso R. A.,
Grass R. N.,
Stark W. J.
Publication year - 2013
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201300925
Subject(s) - cyanide , immunoassay , substrate (aquarium) , materials science , colloidal gold , dissolution , electrode , nanotechnology , chemistry , chromatography , nanoparticle , biology , organic chemistry , antibody , immunology , metallurgy , ecology
Immunoassays are used for detecting protein targets for various applications. Here, a modification of immunoassays to allow a purely electrical detection of the target protein concentration is shown. The modification comprises a β‐D‐glucosidase as reporter enzyme and a cyanogenic glycoside as substrate. The enzymatic reaction produces cyanide in small quantities. For electrical detection of the cyanide, a novel sensor is developed, based on a gold micro wire. The cyanide dissolves the gold wire and changes the electrical resistance of the wire. Monitoring the resistance change allows a quantitative measurement of the target human C‐reactive protein (an inflammatory marker) in blood plasma in the physiological relevant concentration range.