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A Facile Method for Preparation of Tailored Scaffolds for DNA‐Origami
Author(s) -
Erkelenz Michael,
Bauer Dennis M.,
Meyer Rebecca,
Gatsogiannis Christos,
Raunser Stefan,
Saccà Barbara,
Niemeyer Christof M.
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201300701
Subject(s) - dna origami , restriction enzyme , dna , restriction site , cloning (programming) , mutagenesis , sticky and blunt ends , plasmid , endonuclease , computational biology , biology , genetics , mutation , computer science , gene , programming language
A convenient PCR cloning strategy allows one to prepare hundreds of picomoles of circular single‐stranded DNA molecules, which are suitable as scaffolds for the assembly of DNA origami structures. The method is based on a combination of site‐directed mutagenesis and site‐ and ligation‐independent cloning protocols, with simultaneous insertion of a nicking endonuclease restriction site on a double‐stranded plasmid of desired length and sequence.