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An Assessment of the Impact of SiO 2 Nanoparticles of Different Sizes on the Rest/Wake Behavior and the Developmental Profile of Zebrafish Larvae
Author(s) -
Xue JiYang,
Li Xiang,
Sun MingZhu,
Wang YaPing,
Wu Ming,
Zhang ChunYang,
Wang YaNan,
Liu Bo,
Zhang YaoShu,
Zhao Xin,
Feng XiZeng
Publication year - 2013
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201300430
Subject(s) - wake , rest (music) , nanoparticle , zebrafish , materials science , nanotechnology , biology , mechanics , physics , acoustics , biochemistry , gene
In this study, zebrafish larvae are introduced as an in vivo platform to examine the neurotoxicity and developmental toxicity associated with continuous exposure to a concentration gradient of different sizes of SiO 2 nanoparticles (15 nm and 50 nm diameter) to determine the dose effect and size effect of SiO 2 nanoparticle (NP)‐induced toxicity. Bovine serum albumin (BSA‐V) is utilized as a stabilizing agent to prevent coagulation of the SiO 2 nanoparticles. To the best of our knowledge, this study is the first to describe locomotor activity assays linking rest/wake behavioral profiles for the purpose of investigating the neurotoxicity of NPs. In addition, developmental toxicological endpoints including mortality, LC 50 , malformation, and cartilaginous deformity are assessed. The results show a concentration‐dependent increase in behavioral neurotoxicity, mortality, and malformation among larvae treated with the SiO 2 nanoparticles of 15 nm and 50 nm. A comparison of the 15 nm and 50 nm NPs by K‐means clustering analysis demonstrates that the 15 nm NPs have a greater neurotoxic effect than the 50 nm NPs, with the 50 nm NPs exhibiting greater developmental toxicity on the zebrafish larvae than the 15 nm NPs.