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Ammonium and Guanidinium Dendron–Carbon Nanotubes by Amidation and Click Chemistry and their Use for siRNA Delivery
Author(s) -
Battigelli Alessia,
Wang Julie TzuWen,
Russier Julie,
Da Ros Tatiana,
Kostarelos Kostas,
AlJamal Khuloud T.,
Prato Maurizio,
Bianco Alberto
Publication year - 2013
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201300264
Subject(s) - dendrimer , carbon nanotube , click chemistry , chemistry , cationic polymerization , combinatorial chemistry , conjugate , agarose gel electrophoresis , guanidine , nanotechnology , ammonium , cytotoxicity , organic chemistry , materials science , dna , biochemistry , mathematical analysis , mathematics , in vitro
A series of multi‐walled carbon nanotube (MWCNT) conjugates is described, functionalized with different dendrons bearing positive charges at their termini (i.e. ammonium or guanidinium groups). The dendrimeric units are anchored to the nanotube scaffolds using two orthogonal synthetic approaches, amidation and click reactions. The final nanohybrids are characterized by complementary analytical techniques, while their ability to interact with siRNA is investigated by means of agarose gel electrophoresis. The demonstration of the cell uptake capacity, the low cytotoxicity, and the ability of these cationic conjugates to silence cytotoxic genes suggests them to be promising carriers for genetic material.