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A Near‐Infrared Fluorescent Nanosensor (AuC@Urease) for the Selective Detection of Blood Urea
Author(s) -
Nair Lakshmi V.,
Philips Divya S.,
Jayasree Ramapurath S.,
Ajayaghosh Ayyappanpillai
Publication year - 2013
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201300213
Subject(s) - urease , urea , detection limit , chemistry , nanosensor , analyte , fluorescence , chromatography , biochemistry , materials science , nanotechnology , physics , quantum mechanics
The use of a nanosensor (AuC@Urease) based on an NIR‐emitting gold cluster and urease enzyme selectively detects urea in whole blood. The detection is based on an enzyme‐specific conversion of urea to ammonium ions which facilitates pH‐induced aggregation of AuC, leading to fluorescence quenching. This method does not interfere with urease inactive analytes or the autofluorescence of blood as confirmed by comparable urea levels obtained by AuC@Urease and standard clinical methods within an error limit of ±3%.