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Bright Far‐Red/Near‐Infrared Conjugated Polymer Nanoparticles for In Vivo Bioimaging
Author(s) -
Ding Dan,
Liu Jie,
Feng Guangxue,
Li Kai,
Hu Yong,
Liu Bin
Publication year - 2013
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201300171
Subject(s) - fluorescence , conjugated system , in vivo , alexa fluor , nanoparticle , chemistry , polyethylene glycol , peg ratio , polymer , quantum yield , nuclear chemistry , rhodamine , near infrared spectroscopy , polymerization , photochemistry , materials science , nanotechnology , organic chemistry , microbiology and biotechnology , finance , quantum mechanics , economics , biology , physics
A highly emissive far‐red/near‐infrared (FR/NIR) fluorescent conjugated polymer (CP), poly[(9,9‐dihexylfluorene)‐ co ‐2,1,3‐benzothiadiazole‐ co ‐4,7‐di(thiophen‐2‐yl)‐2,1,3‐benzothiadiazole] (PFBTDBT10) is designed and synthesized via Suzuki polymerization. Formulation of PFBTDBT10 using 1,2‐distearoyl‐ sn ‐glycero‐3‐phosphoethanolamine‐ N ‐[methoxy(polyethylene glycol)‐2000] (DSPE‐PEG 2000 ) and DSPE‐PEG 5000 ‐folate as the encapsulation matrix yielded CP‐loaded DSPE‐PEG‐folic acid nanoparticles (CPDP‐FA NPs) with bright FR/NIR fluorescence (27% quantum yield) and a large Stoke's shift of 233 nm in aqueous solution. CPDP‐FA NPs show improved thermal/photostabilities and larger Stoke's shifts as compared to commercially available quantum dots (Qdot 655) and organic dyes such as Alexa Fluor 555 and Rhodamine 6G. In vivo studies of CPDP‐FA NPs on a hepatoma H22 tumor‐bearing mouse model reveal that they could serve as an efficient FR/NIR fluorescent probe for targeted in vivo fluorescence imaging and cancer detection in a high contrast and specific manner. Together with the negligible in vivo toxicity, CPDP‐FA NPs are promising FR/NIR fluorescent probes for future in vivo applications.