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Enzyme Catalysis: Enzymatic Growth of Quantum Dots: Applications to Probe Glucose Oxidase and Horseradish Peroxidase and Sense Glucose (Small 22/2012)
Author(s) -
Saa Laura,
Pavlov Valeri
Publication year - 2012
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201290120
Subject(s) - glucose oxidase , horseradish peroxidase , chemistry , peroxidase , cadmium sulfide , enzyme , quantum dot , fluorescence , nanoparticle , catalysis , photochemistry , nuclear chemistry , biochemistry , nanotechnology , inorganic chemistry , materials science , physics , quantum mechanics
Three innovative assays have been developed for the detection of the enzymatic activities of glucose oxidase (GOx) and horseradish peroxidase (HRP) by the generation of CdS quantum dots (QDs) in situ using an unconventional enzymatic reaction. By employing 1‐thiob‐D‐glucose and sodium thiosulfate as enzymatic substrates, sulfide ions are generated which, in the presence of cadmium ions, yield fluorescent CdS nanoparticles. On page 3449 , L. Saa and V. Pavlov show how the combination of GOx with HRP allows quantification of the glucose in human plasma by following the growth of the fluorescent QDs.