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Affinity Separation: M13 Bacteriophage‐Activated Superparamagnetic Beads for Affinity Separation (Small 15/2012)
Author(s) -
Muzard Julien,
Platt Mark,
Lee Gil U.
Publication year - 2012
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201290083
Subject(s) - bacteriophage , superparamagnetism , bead , monolayer , materials science , nanotechnology , chemistry , chromatography , biochemistry , physics , escherichia coli , magnetization , quantum mechanics , magnetic field , composite material , gene
M13 bacteriophage virons are immobilized on superparamagnetic beads by G. U. Lee and co‐workers, either through the pIII proteins on the ends of the phages or by the pVIII proteins on the sides of the phages. These two immobilization schemes, shown in detail on page 2403 , result in an end‐on phage–superparamagnetic bead assembly or a monolayer of phage covering the superparamagnetic beads in a side‐on orientation, respectively.