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NIR‐Emitting Quantum Dot‐Encoded Microbeads through Membrane Emulsification for Multiplexed Immunoassays
Author(s) -
Wang Xiebing,
Wang Gang,
Li Wanwan,
Zhao Bingxia,
Xing Bin,
Leng Yuankui,
Dou Hongjing,
Sun Kang,
Shen Lisong,
Yuan Xiangliang,
Li Jiyu,
Sun Kun,
Han Junsong,
Xiao Huasheng,
Li Yue,
Huang Peng,
Chen Xiaoyuan
Publication year - 2013
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201203156
Subject(s) - materials science , quantum dot , photoluminescence , hbsag , multiplex , membrane emulsification , nanotechnology , membrane , optoelectronics , hepatitis b virus , chemistry , virology , virus , bioinformatics , biochemistry , biology
NIR‐emitting CdSeTe/CdS/ZnS core/shell/shell QD‐encoded microbeads are combined with common flow cytometry with one laser for multiplexed detection of hepatitis B virus (HBV). A facile one‐pot synthetic route is developed to prepare CdSeTe/CdS/ZnS core/shell/shell QDs with high photoluminescence quantum yield and excellent stability in liquid paraffin, and a Shirasu porous glass (SPG) membrane emulsification technique is applied to incorporate the QDs into polystyrene–maleic anhydride (PSMA) microbeads to obtain highly fluorescent QD‐encoded microbeads. The relatively wide NIR photoluminescence full width half maximum of the CdSeTe/CdS/ZnS QDs is used to develop a ‘single wavelength’ encoding method to obtain different optical codes by changing the wavelengh and emission intensity of the QDs incorporated into the microbeads. Moreover, a detection platform combining NIR‐emitting CdSeTe/CdS/ZnS QD‐encoded microbeads and Beckman Coulter FC 500 flow cytometry with one laser of 488 nm is successfully used to conduct a 2‐plex hybridization assay for hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), and a 3‐plex hybridization assay for hepatitis B surface antibody (HBsAb), hepatitis B e antibody (HBeAb), and hepatitis B core antibody (HBcAb), which suggests the promising application of NIR QD‐encoded microbeads for multiplex immunoassays.