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A Molecular Beacon‐Based Signal‐Off Surface‐Enhanced Raman Scattering Strategy for Highly Sensitive, Reproducible, and Multiplexed DNA Detection
Author(s) -
Wei Xinpan,
Su Shao,
Guo Yuanyuan,
Jiang Xiangxu,
Zhong Yiling,
Su Yuanyuan,
Fan Chunhai,
Lee ShuitTong,
He Yao
Publication year - 2013
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201202914
Subject(s) - molecular beacon , raman scattering , oligonucleotide , multiplexing , nanotechnology , signal (programming language) , pipeline (software) , materials science , raman spectroscopy , dna , oligomer restriction , computer science , chemistry , physics , telecommunications , optics , biochemistry , programming language
Stem‐loop oligonucleotides tagged with organic dyes are employed as capture and reporter probes in a molecular beacon‐based signal‐off surface‐ enhanced Raman scattering (SERS) strategy. DNA strands are immobilized on a gold nanoparticle‐decorated silicon nanowire array (AuNPs@SiNWAr). DNA with a concentration down to ≈10 f M is detected under irradiation from a low‐power laser, which is better than or comparable to that detected by signal‐on SERS methods. The signal‐off strategy allows identification of single‐base mismatches and detection of multiplexed DNA.