Premium
Graphene‐Induced Adsorptive and Optical Artifacts During In Vitro Toxicology Assays
Author(s) -
Creighton Megan A.,
RangelMendez J. Rene,
Huang Jiaxing,
Kane Agnes B.,
Hurt Robert H.
Publication year - 2013
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201202625
Subject(s) - graphene , fluorescence , biomolecule , quenching (fluorescence) , in vitro , nanotechnology , in vitro toxicology , materials science , viability assay , biophysics , chemistry , biochemistry , biology , optics , physics
Graphene materials can deplete essential micronutrients from cell culture media and potentially interfere with probe dyes used during in vitro analysis of cell viability and function by (1) adsorbing dyes before they can interact with their target biomolecule, (2) quenching fluorescence after the interaction, and/or (3) absorbing or scattering light, which are issues for both colorimetric and fluorescent assays.