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Nanotopographical Cues Augment Mesenchymal Differentiation of Human Embryonic Stem Cells
Author(s) -
Kingham Emmajayne,
White Kate,
Gadegaard Nikolaj,
Dalby Matthew J.,
Oreffo Richard O. C.
Publication year - 2013
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201202340
Subject(s) - mesenchymal stem cell , embryonic stem cell , microbiology and biotechnology , embryoid body , cellular differentiation , progenitor cell , stem cell , directed differentiation , transplantation , biology , stromal cell , adult stem cell , induced pluripotent stem cell , cancer research , medicine , biochemistry , gene
The production of bone‐forming osteogenic cells for research purposes or transplantation therapies remains a significant challenge. Using planar polycarbonate substrates lacking in topographical cues and substrates displaying a nanotopographical pattern, mesenchymal differentiation of human embryonic stem cells is directed in the absence of chemical factors and without induction of differentiation by embryoid body formation. Cells incubated on nanotopographical substrates show enhanced expression of mesenchymal or stromal markers and expression of early osteogenic progenitors at levels above those detected in cells on planar substrates in the same basal media. Evidence of epithelial‐to‐mesenchymal transition during substrate differentiation and DNA methylation changes akin to chemical induction are also observed. These studies provide a suitable approach to overcome regenerative medical challenges and describe a defined, reproducible platform for human embryonic stem cell differentiation.