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Force‐Controlled Fluidic Injection into Single Cell Nuclei
Author(s) -
GuillaumeGentil Orane,
Potthoff Eva,
Ossola Dario,
Dörig Pablo,
Zambelli Tomaso,
Vorholt Julia A.
Publication year - 2013
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201202276
Subject(s) - microfluidics , fluidics , nanotechnology , biomolecule , atomic force microscopy , cytoplasm , membrane , materials science , biophysics , chemistry , engineering , biology , aerospace engineering , biochemistry
Surpassing the physical barriers of the cytoplasmic and nuclear membranes to deliver biomolecules directly into cell nuclei offers opportunities to investigate dynamic processes in living cells. The potential of atomic force microscopy coupled to microfluidics (FluidFM) for volume‐controlled intranuclear delivery is demonstrated, whereby minimally invasive microchanneled probes are remotely driven with high spatiotemporal resolution.