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Quantum Dot‐Based Thermal Spectroscopy and Imaging of Optically Trapped Microspheres and Single Cells
Author(s) -
HaroGonzález Patricia,
Ramsay William T.,
Maestro Laura Martinez,
del Rosal Blanca,
SantacruzGomez Karla,
del Carmen Iglesiasde la Cruz Maria,
SanzRodríguez Francisco,
Chooi Jing Yuang,
Sevilla Paloma Rodriguez,
Bettinelli Marco,
Choudhury Debaditya,
Kar Ajoy K.,
Solé José García,
Jaque Daniel,
Paterson Lynn
Publication year - 2013
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201201740
Subject(s) - quantum dot , materials science , luminescence , spectroscopy , trapping , laser , thermal , optoelectronics , absorption (acoustics) , wavelength , radiation , optics , molecular physics , chemistry , physics , ecology , quantum mechanics , meteorology , composite material , biology
Laser‐induced thermal effects in optically trapped microspheres and single cells are investigated by quantum dot luminescence thermometry. Thermal spectroscopy has revealed a non‐localized temperature distribution around the trap that extends over tens of micrometers, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. The experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This is corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. This quantum dot luminescence thermometry demonstrates that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43 °C), thus, avoiding cell damage.