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Nanostructured Superhydrophobic Substrates Trigger the Development of 3D Neuronal Networks
Author(s) -
Limongi Tania,
Cesca Fabrizia,
Gentile Francesco,
Marotta Roberto,
Ruffilli Roberta,
Barberis Andrea,
Dal Maschio Marco,
Petrini Enrica Maria,
Santoriello Stefania,
Benfenati Fabio,
Di Fabrizio Enzo
Publication year - 2013
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201201377
Subject(s) - neurite , nanotechnology , materials science , pillar , regeneration (biology) , scanning electron microscope , confocal microscopy , neuroscience , chemistry , microbiology and biotechnology , biology , engineering , biochemistry , structural engineering , in vitro , composite material
The generation of 3D networks of primary neurons is a big challenge in neuroscience. Here, a novel method is presented for a 3D neuronal culture on superhydrophobic (SH) substrates. How nano‐patterned SH devices stimulate neurons to build 3D networks is investigated. Scanning electron microscopy and confocal imaging show that soon after plating neurites adhere to the nanopatterned pillar sidewalls and they are subsequently pulled between pillars in a suspended position. These neurons display an enhanced survival rate compared to standard cultures and develop mature networks with physiological excitability. These findings underline the importance of using nanostructured SH surfaces for directing 3D neuronal growth, as well as for the design of biomaterials for neuronal regeneration.