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Detection of Circulating Cancer Cells Using Electrocatalytic Gold Nanoparticles
Author(s) -
Maltezda Costa Marisa,
de la EscosuraMuñiz Alfredo,
Nogués Carme,
Barrios Lleonard,
Ibáñez Elena,
Merkoçi Arben
Publication year - 2012
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201201205
Subject(s) - colloidal gold , cancer cell , nanoparticle , electrochemistry , suspension (topology) , materials science , nanotechnology , suspension culture , membrane , circulating tumor cell , electrochemical cell , cancer , cell , detection limit , chemistry , biophysics , cell culture , chromatography , electrode , biochemistry , metastasis , biology , genetics , mathematics , homotopy , pure mathematics
A rapid cancer cell detection and quantification assay, based on the electrocatalytic properties of gold nanoparticles towards the hydrogen evolution reaction, is described. The selective labeling of cancer cells is performed in suspension, allowing a fast interaction between the gold nanoparticle labels and the target proteins expressed at the cell membrane. The subsequent electrochemical detection is accomplished with small volumes of sample and user‐friendly equipment through a simple electrochemical method that generates a fast electrochemical response used for the quantification of nanoparticle‐labeled cancer cells. The system establishes a selective cell‐detection assay capable of detecting 4 × 10 3 cancer cells in suspension that can be extended to several other cells detection scenarios.