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Fluorescent Silica Nanoparticles Improve Optical Imaging of Stem Cells Allowing Direct Discrimination between Live and Early‐Stage Apoptotic Cells
Author(s) -
Accomasso Lisa,
Rocchietti Elisa Cibrario,
Raimondo Stefania,
Catalano Federico,
Alberto Gabriele,
Giannitti Andrea,
Minieri Valentina,
Turinetto Valentina,
Orlando Luca,
Saviozzi Silvia,
Caputo Giuseppe,
Geuna Stefano,
Martra Gianmario,
Giachino Claudia
Publication year - 2012
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201200882
Subject(s) - mesenchymal stem cell , confocal microscopy , stem cell , flow cytometry , embryonic stem cell , confocal , materials science , microbiology and biotechnology , biocompatibility , nanotechnology , biophysics , fluorescence microscope , cell , fluorescence , chemistry , biology , biochemistry , geometry , mathematics , physics , quantum mechanics , metallurgy , gene
Highly bright and photostable cyanine dye‐doped silica nanoparticles, IRIS Dots, are developed, which can efficiently label human mesenchymal stem cells (hMSCs). The application procedure used to label hMSCs is fast (2 h), the concentration of IRIS Dots for efficient labeling is low (20 μg mL −1 ), and the labeled cells can be visualized by flow cytometry, confocal microscopy, and transmission electron microscopy. Labeled hMSCs are unaffected in their viability and proliferation, as well as stemness surface marker expression and differentiation capability into osteocytes. Moreover, this is the first report that shows nonfunctionalized IRIS Dots can discriminate between live and early‐stage apoptotic stem cells (both mesenchymal and embryonic) through a distinct external cell surface distribution. On the basis of biocompatibility, efficient labeling, and apoptotic discrimination potential, it is suggested that IRIS Dots can serve as a promising stem cell tracking agent.