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Kidney Podocytes as Specific Targets for cyclo(RGDfC)‐Modified Nanoparticles
Author(s) -
Pollinger Klaus,
Hennig Robert,
Breunig Miriam,
Tessmar Joerg,
Ohlmann Andreas,
Tamm Ernst R.,
Witzgall Ralph,
Goepferich Achim
Publication year - 2012
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201200733
Subject(s) - podocyte , receptor , confocal microscopy , integrin , microbiology and biotechnology , kidney , biophysics , in vivo , ex vivo , vesicle , chemistry , materials science , nanotechnology , in vitro , membrane , biology , biochemistry , endocrinology , proteinuria
Renal nanoparticle passage opens the door for targeting new cells like podocytes, which constitute the exterior part of the renal filter. When cyclo(RGDfC)‐modified Qdots are tested on isolated primary podocytes for selective binding to the αvβ3 integrin receptor a highly cell‐ and receptor‐specific binding can be observed. In displacement experiments with free cyclo(RGDfC) IC 50 values of 150 nM for αvβ3 integrin over‐expressing U87‐MG cells and 60 nM for podocytes are measured. Confocal microscopy shows a cellular Qdot uptake into vesicle‐like structures. Our ex vivo study gives clear evidence that, after renal filtration, nanoparticles can be targeted to podocyte integrin receptors in the future. This could be a highly promising approach for future therapy and diagnostics of podocyte‐associated diseases.