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Graphene‐Oxide‐Based Immunosensing through Fluorescence Quenching by Peroxidase‐Catalyzed Polymerization
Author(s) -
Lim Seong Yoon,
Ahn Junhyoung,
Lee Joon Seok,
Kim MinGon,
Park Chan Beum
Publication year - 2012
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201200379
Subject(s) - graphene , biomolecule , oxide , immunoassay , fluorescence , polymerization , peroxidase , nanotechnology , quenching (fluorescence) , materials science , chemistry , biochemistry , enzyme , polymer , immunology , biology , organic chemistry , antibody , physics , quantum mechanics
A graphene oxide (GO)‐based immunosensor is developed for the detection of interleukin‐5 (IL‐5), a key cytokine associated with asthma pathology and eosinophilia. The immunosensing platform utilizes the innate fluorescence of GO, not demanding biomolecules labeled with fluorescent dyes. The GO‐based immunoassay exhibits high specificity for IL‐5 among other cytokines and is not affected by nonspecific proteins in human serum.