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High‐Speed Tracking of Murine Cardiac Stem Cells by Harmonic Nanodoublers
Author(s) -
Magouroux Thibaud,
Extermann Jerome,
Hoffmann Pernilla,
Mugnier Yannick,
Le Dantec Ronan,
Jaconi Marisa E.,
Kasparian Christelle,
Ciepielewski Daniel,
Bonacina Luigi,
Wolf JeanPierre
Publication year - 2012
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201200366
Subject(s) - embryonic stem cell , stem cell , nonlinear system , second harmonic generation , nanoparticle , materials science , regeneration (biology) , regenerative medicine , contraction (grammar) , biophysics , nanotechnology , biomedical engineering , biological system , optics , microbiology and biotechnology , chemistry , biology , physics , engineering , biochemistry , laser , quantum mechanics , endocrinology , gene
Potassium niobate nonlinear nanoparticles are used for the first time to monitor the evolution of embryonic stem cells (ESC) by second harmonic microscopy. These particles feature the complete absence of photo‐bleaching and unlimited excitation wavelength flexibility. The potential of this approach is made evident for tissue‐regeneration studies and applications, by capturing a high‐speed movie of ESC‐derived cardiomyocytes autonomously beating within a cluster. Time‐resolved data are analyzed to retrieve 3D information of the contraction pattern at the cellular level.