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Cancer Prognostics by Direct Detection of p53‐Antibodies on Gold Surfaces by Impedance Measurements
Author(s) -
PratsAlfonso Elisabet,
Sisquella Xavier,
Zine Nadia,
Gabriel Gemma,
Guimerà Anton,
del Campo F. Javier,
Villa Rosa,
Eisenberg Adam H.,
Mrksich Milan,
Errachid Abdelhamid,
Aguiló Jordi,
Albericio Fernando
Publication year - 2012
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201102724
Subject(s) - materials science , biosensor , biomarker , nanotechnology , cancer , microelectrode , antibody , cancer biomarkers , biomedical engineering , chemistry , electrode , medicine , immunology , biochemistry
The identification and measurement of biomarkers is critical to a broad range of methods that diagnose and monitor many diseases. Serum auto‐antibodies are rapidly becoming interesting targets because of their biological and medical relevance. This paper describes a highly sensitive, label‐free approach for the detection of p53‐antibodies, a prognostic indicator in ovarian cancer as well as a biomarker in the early stages of other cancers. This approach uses impedance measurements on gold microelectrodes to measure antibody concentrations at the picomolar level in undiluted serum samples. The biosensor shows high selectivity as a result of the optimization of the epitopes responsible for the detection of p53‐antibodies and was validated by several techniques including microcontact printing, self‐assembled‐monolayer desorption ionization (SAMDI) mass spectrometry, and adhesion pull‐off force by atomic force microscopy (AFM). This transduction method will lead to fast and accurate diagnostic tools for the early detection of cancer and other diseases.