Premium
Improved Photodynamic Cancer Treatment by Folate‐Conjugated Polymeric Micelles in a KB Xenografted Animal Model
Author(s) -
Syu WeiJhe,
Yu HsiuPing,
Hsu ChiaYen,
Rajan Yeasudhasan Christu,
Hsu YuanHung,
Chang YuanChia,
Hsieh WenYuan,
Wang ChauHui,
Lai PingShan
Publication year - 2012
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201102695
Subject(s) - photosensitizer , phototoxicity , photodynamic therapy , micelle , chlorin , conjugated system , folate receptor , in vivo , chemistry , biophysics , in vitro , photochemistry , cancer cell , cancer , organic chemistry , biochemistry , medicine , biology , polymer , microbiology and biotechnology , aqueous solution
Photodynamic therapy (PDT) is a light‐induced chemical reaction that produces localized tissue damage for the treatment of cancers and various nonmalignant conditions. In the clinic, patients treated with PDT should be kept away from direct sunlight or strong indoor lighting to avoid skin phototoxicity. In a previous study, it was demonstrated that the skin phototoxicity of meta ‐ tetra (hydroxyphenyl)chlorin (m‐THPC), a photosensitizer used in the clinic, can be significantly reduced after micellar encapsulation; however, no improvement in antitumor efficacy was observed. In this work, a folate‐conjugated polymeric m‐THPC delivery system is developed for improving tumor targeting of the photosensitizer, preventing photodamage to the healthy tissue, and increasing the effectiveness of the photosensitizers. The results demonstrate that folate‐conjugated m‐THPC‐loaded micelles with particle sizes around 100 nm are taken up and accumulated by folate receptor‐overexpressed KB cells in vitro and in vivo, and their PDT has no significant adverse effects on the body weight of mice. After an extended delivery time, a single dose of folate‐conjugated m‐THPC‐loaded micelles has higher antitumor effects (tumor growth inhibition = 92%) through inhibition of cell proliferation and reduction of vessel density than free m‐THPC or m‐THPC‐loaded micelles at an equivalent m‐THPC concentration of 0.3 mg kg −1 after irradiation. Furthermore, folate‐conjugated m‐THPC‐loaded micelles at only 0.2 mg kg −1 m‐THPC have a similar antitumor efficacy to m‐THPC or m‐THPC‐loaded micelles with the m‐THPC concentration at 0.3 mg kg −1 , which indicates that the folate conjugation on the micellar photosensitizer apparently reduces the requirement of m‐THPC for PDT. Thus, folate‐conjugated m‐THPC‐loaded micelles with improved selectivity via folate–folate receptor interactions have the potential to reduce, not only the skin photosensitivity, but also the drug dose requirement for clinical PDT.