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Silica‐Modified Oligonucleotide−Gold Nanoparticle Conjugate Enables Closed‐Tube Colorimetric Polymerase Chain Reaction
Author(s) -
Wong Jacky K. F.,
Yip Shea Ping,
Lee Thomas M. H.
Publication year - 2012
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201101925
Subject(s) - oligonucleotide , conjugate , colloidal gold , polymerase chain reaction , nanoparticle , combinatorial chemistry , nanotechnology , dna , computer science , chemistry , materials science , biochemistry , mathematics , gene , mathematical analysis
A facile silica coating significantly enhances the thermal stability and polymerase chain reaction (PCR) compatibility of oligonucleotide−gold nanoparticle conjugates, thus enabling colorimetric detection of PCR results in a closed‐tube format. This method is specific, sensitive, and generally applicable. Its simplicity, visual readout, and carryover contamination‐free features hold promise for point‐of‐care or on‐site DNA testing.