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Design, Assembly, and Activity of Antisense DNA Nanostructures
Author(s) -
Keum JungWon,
Ahn JinHo,
Bermudez Harry
Publication year - 2011
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201101804
Subject(s) - dna , dna nanotechnology , nanotechnology , nanostructure , gene silencing , microbiology and biotechnology , gene , chemistry , biology , computational biology , biophysics , materials science , biochemistry
Abstract Discrete DNA nanostructures allow simultaneous features not possible with traditional DNA forms: encapsulation of cargo, display of multiple ligands, and resistance to enzymatic digestion. These properties suggested using DNA nanostructures as a delivery platform. Here, DNA pyramids displaying antisense motifs are shown to be able to specifically degrade mRNA and inhibit protein expression in vitro, and they show improved cell uptake and gene silencing when compared to linear DNA. Furthermore, the activity of these pyramids can be regulated by the introduction of an appropriate complementary strand. These results highlight the versatility of DNA nanostructures as functional devices.