Premium
Orthogonal Protein Decoration of DNA Nanostructures
Author(s) -
Meyer Rebecca,
Niemeyer Christof M.
Publication year - 2011
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201101365
Subject(s) - dna , dna origami , nanotechnology , dna nanotechnology , streptavidin , nanostructure , chemistry , materials science , computational biology , biochemistry , biology , biotin
The development of robust DNA–protein coupling techniques is mandatory for applications of DNA nanostructures in biomedical diagnostics, fundamental biochemistry, and other fields in biomolecular nanosciences. The use of self‐labeling fusion proteins, which are orthogonal to biotin–streptavidin and antibody–antigen interactions, is described for the site‐selective protein decoration of two exemplary DNA nanostructures: a four‐way junction X‐tile motif and a 3D DNA tetrahedron. Multifunctional DNA superstructures bearing up to four different proteins are generated and characterized by electrophoresis and microplate‐based functionality assays. Steric and electrostatic interactions are identified as critical parameters controlling the efficiency of DNA–protein ligation. The results indicate that this method is versatile and broadly applicable, not only for the functionalization of DNA architectures but also for the site‐specific decoration of other molecular materials and devices containing several different proteins.