Premium
Parameters Affecting the Efficient Delivery of Mesoporous Silica Nanoparticle Materials and Gold Nanorods into Plant Tissues by the Biolistic Method
Author(s) -
MartinOrtigosa Susana,
Valenstein Justin S.,
Sun Wei,
Moeller Lorena,
Fang Ning,
Trewyn Brian G.,
Lin Victor S.Y.,
Wang Kan
Publication year - 2012
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201101294
Subject(s) - nanotechnology , nanoparticle , nanorod , materials science , colloidal gold , mesoporous silica , in vivo , mesoporous material , chemistry , microbiology and biotechnology , biology , biochemistry , catalysis
Applying nanotechnology to plant science requires efficient systems for the delivery of nanoparticles (NPs) to plant cells and tissues. The presence of a cell wall in plant cells makes it challenging to extend the NP delivery methods available for animal research. In this work, research is presented which establishes an efficient NP delivery system for plant tissues using the biolistic method. It is shown that the biolistic delivery of mesoporous silica nanoparticle (MSN) materials can be improved by increasing the density of MSNs through gold plating. Additionally, a DNA‐coating protocol is used based on calcium chloride and spermidine for MSN and gold nanorods to enhance the NP‐mediated DNA delivery. Furthermore, the drastic improvement of NP delivery is demonstrated when the particles are combined with 0.6 μm gold particles during bombardment. The methodology described provides a system for the efficient delivery of NPs into plant cells using the biolistic method.