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Targeted Delivery of Polyoxometalate Nanocomposites
Author(s) -
Geisberger Georg,
Paulus Susann,
Gyenge Emina Besic,
Maake Caroline,
Patzke Greta R.
Publication year - 2011
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201101264
Subject(s) - polyoxometalate , chitosan , nanocomposite , nanoparticle , confocal microscopy , fluorescein isothiocyanate , transmission electron microscopy , nanotechnology , materials science , fluorescein , cytotoxicity , isothiocyanate , drug delivery , fluorescence , confocal , nuclear chemistry , biophysics , chemistry , organic chemistry , biochemistry , catalysis , physics , quantum mechanics , microbiology and biotechnology , in vitro , biology , geometry , mathematics
Polyoxometalate/carboxymethyl chitosan nanocomposites with an average diameter of 130 nm are synthesized and labeled with fluorescein isothiocyanate (FITC) for a combined drug‐carrier and cellular‐monitoring approach. [Eu( β 2 ‐SiW 11 O 39 ) 2 ] 13− /CMC nanospheres as a representative example do not display cytotoxicity for POM concentrations up to 2 mg mL −1 . Cellular uptake of fluoresecently labelled {EuSiW 11 O 39 }/FITC‐CMC nanoparticles is monitored with confocal laser scanning microscopy. Nanoparticle uptake occurs after incubation times of around 1 h and no cyctotoxic effects are observed upon prolonged treatment. The preferential location of the POM/CMC nanocomposites in the perinuclear region is furthermore verified with transmission electron microscopy investigations on unlabeled nanoparticles. Therefore, this approach is a promising dual strategy for the safe cellular transfer and monitoring of bioactive POMs.