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The Exocytosis of Fluorescent Nanodiamond and Its Use as a Long‐Term Cell Tracker
Author(s) -
Fang ChiaYi,
Vaijayanthimala V.,
Cheng ChiAn,
Yeh ShihHua,
Chang ChingFang,
Li ChungLeung,
Chang HuanCheng
Publication year - 2011
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201101233
Subject(s) - exocytosis , hela , stromal cell , cell culture , cell , confocal microscopy , microbiology and biotechnology , fluorescence microscope , cancer cell , biophysics , fluorescence , chemistry , materials science , biology , biochemistry , cancer research , cancer , membrane , genetics , physics , quantum mechanics
Fluorescent nanodiamond (FND) has excellent biocompatibility and photostability, making it well suited for long‐term labeling and tracking of cancer and stem cells. To prove the concept, the exocytosis of FND particles (size ≈100 nm) from three cell lines—HeLa cervical cancer cells, 3T3‐L1 pre‐adipocytes, and 489‐2.1 multipotent stromal cells—is studied in detail. FND labeling is performed by incubating the cells in a serum‐free medium containing 80 μg mL −1 FND for 4 h. No significant alteration in growth or proliferation of the FND‐labeled cells, including the multipotent stromal cells, is observed for up to 8 days. Flow cytometric analysis, in combination with parallel cell doubling‐time measurements, indicates that there is little (≈15% or less) excretion of the endocytosed FND particles after 6 days of labeling for both HeLa and 489‐2.1 cells, but exocytosis occurs more readily (up to 30%) for 3T3‐L1 preadipocytes. A comparative experiment with FND and the widely used dye, carboxyfluorescein diacetate succinimidyl ester, demonstrates that the nanoparticle platform is a promising alternate probe for long‐term cell labeling and tracking applications.