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Synthesis of Silver Nanoparticle–Hollow Titanium Phosphate Sphere Hybrid as a Label for Ultrasensitive Electrochemical Detection of Human Interleukin‐6
Author(s) -
Peng Juan,
Feng LiNa,
Ren ZhongJie,
Jiang LiPing,
Zhu JunJie
Publication year - 2011
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201101210
Subject(s) - materials science , detection limit , dispersity , biocompatibility , silver nanoparticle , nanoparticle , titanium , nanotechnology , electrochemistry , electrode , chemical engineering , chromatography , chemistry , polymer chemistry , engineering , metallurgy
A silver nanoparticle–hollow titanium phosphate sphere (AgNP–TiP) hybrid is successfully synthesized and used as a label for electrochemical detection of human interleukin‐6 (IL‐6). Hollow TiP spheres with a diameter of 430 nm and an average thickness of 40 nm are synthesized by a template approach. The AgNPs are incorporated in situ into the TiP shell via an exchange process. The as‐prepared AgNP–TiP hybrid shows outstanding biocompatibility, good dispersity and solubility in water, and high silver loading properties (289.2 mg of silver in 1.0 g of TiP). These advantages make the AgNP–TiP hybrid an effective candidate as an amplification label in immunoassay systems. Herein, the as‐prepared AgNP–TiP hybrid is attached to a signal antibody (Ab 2 ) to produce Ab 2 –AgNP–TiP labels in the fabrication of an electrochemical immunosensor. The nanoparticle‐based amplification labels, upon coupling with a magnetic sensing array, give rise to an extremely sensitive response to IL‐6 in a linear range of 0.0005–10 ng mL −1 with a detection limit of 0.1 pg mL −1 . The proposed sensor exhibits high specificity, good reproducibility, and long‐term stability, and may be a promising technique for protein and DNA detection.