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Nanodiamond as a Vector for siRNA Delivery to Ewing Sarcoma Cells
Author(s) -
Alhaddad Anna,
Adam MariePierre,
Botsoa Jacques,
Dantelle Géraldine,
Perruchas Sandrine,
Gacoin Thierry,
Mansuy Christelle,
Lavielle Solange,
Malvy Claude,
Treussart François,
Bertrand JeanRémi
Publication year - 2011
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201101193
Subject(s) - nanodiamond , internalization , nucleic acid , small interfering rna , in vivo , rna , transfection , cell , chemistry , rna interference , gene silencing , biophysics , microbiology and biotechnology , nanotechnology , materials science , diamond , biochemistry , biology , gene , organic chemistry
The ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) into Ewing sarcoma cells is investigated with a view to the possibility of in‐vivo anticancer nucleic‐acid drug delivery. siRNA is adsorbed onto NDs that are coated with cationic polymer. Cell uptake of NDs is demonstrated by taking advantage of the NDs’ intrinsic fluorescence from embedded color‐center defects. Cell toxicity of these coated NDs is shown to be low. Consistent with the internalization efficacy, a specific inhibition of EWS/Fli‐1 gene expression is shown at the mRNA and protein level by the ND‐vectorized siRNA in a serum‐containing medium.