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Combining a Nanowire SERRS Sensor and a Target Recycling Reaction for Ultrasensitive and Multiplex Identification of Pathogenic Fungi
Author(s) -
Yoo Seung Min,
Kang Taejoon,
Kang Hyungchang,
Lee Hyoban,
Kang Mijeong,
Lee Sang Yup,
Kim Bongsoo
Publication year - 2011
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201100633
Subject(s) - exonuclease , multiplex , exonuclease iii , dna , detection limit , genomic dna , pathogen , materials science , polymerase chain reaction , raman scattering , multiplex polymerase chain reaction , nanotechnology , chemistry , raman spectroscopy , biology , gene , microbiology and biotechnology , biochemistry , polymerase , genetics , chromatography , physics , optics , escherichia coli
Development of a rapid, sensitive, and multiplex pathogen DNA sensor enables early diagnosis and, subsequently, the proper treatment of infectious diseases, increasing the possibility to save the lives of infected patients. Here, the development of an ultrasensitive and multiplex pathogen DNA detection method that combines a patterned Au nanowire (NW)‐on‐film surface‐enhanced resonance Raman scattering (SERRS) sensor with an exonuclease III‐assisted target DNA recycling reaction is reported. Multiple probe DNAs are added to the target DNA solution, and among them, only the complementary probe DNA is selectively digested by exonuclease III, resulting in the decrease in its concentration. The digestion process is repeated by recycling of target DNAs. The decrease of the complementary probe DNA concentration is detected by SERRS. Combining the high sensitivity of the NW‐on‐film sensor and the target recycling reaction significantly improves DNA detection performance, resulting in the detection limit of 100 f M corresponding to 3 amole. By positioning Au NWs at specific addresses, multiple pathogen DNAs can be identified in a single step. Clinical sample tests with multiple genomic DNAs of pathogens show the potential of this sensor for practical diagnosis of infectious diseases.