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Wet Nanoscale Imaging and Testing of Polymersomes
Author(s) -
Battaglia Giuseppe,
LoPresti Caterina,
Massignani Marzia,
Warren Nicholas J.,
Madsen Jeppe,
Forster Simon,
Vasilev Cvetilin,
Hobbs Jamie K.,
Armes Steven P.,
Chirasatitsin Somoyot,
Engler Adam J.
Publication year - 2011
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201100511
Subject(s) - polymersome , biotinylation , nanotechnology , nanoscopic scale , streptavidin , materials science , characterization (materials science) , atomic force microscopy , nanoparticle , computer science , copolymer , chemistry , biotin , polymer , amphiphile , composite material , biochemistry
A facile methodology for the immobilization of polymersomes is demonstrated using ad‐hoc synthesized, biotinylated copolymers or commercially available biotinylated phospholipids on streptavidin‐decorated substrates. This allows the imaging of polymersomes using high‐resolution techniques such as stimulated emission depletion microscopy and atomic force microscopy. This latter allows the gathering of other important functional parameters such as mechanical properties and surface topology, enabling characterization of these water‐borne nanoparticles in wet conditions and hence in their natural environment.